Техники и методи за детекција и дијагноза на орални премалигни и малигни лезии

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Бруно Николовски
Даница Поповиќ-Моневска
Ана Миновска
Мирјана Поповска
Вера Радојкова-Николовска


ОСНОВА: Оралните премалигни и малигни лезии бележат тенденција на доцна детекција, која е на штета на пациентите поради високите стапки на морбидитет и морталитет.. Токму затоа е важно раното дијагностицирање на овие нарушувања и заболувања.

ЦЕЛ: Целта на овој труд е да ги евалуираме дијагностичките средства и методи за детекција на премалигните и малигни орални лезии.

МАТЕРИЈАЛ И МЕТОДИ: За изработка на овој труд, спроведовме автоматско детално пребарување на интернет базата PubMed за периодот од 1966 година до 2014 година. Клучни зборови според кои го изведовме пребарувањето беа: “орални преканцерози, орален карцином, визуелни средства и молекуларни биолошки маркери”. Ги вклучивме и извештаите кои беа рецензирани и прифатени за објавување, а сеуште неиспечатени. Ги исклучивме статиите кои беа известувања од експерти, како и оние кои не беа од англиско издание или не беа изведени на хумани субјекти.

РЕЗУЛТАТИ: Од најчесто употребуваните техники и дијагностички тестови, виталните ткивни боења покажуваат сензитивност од 93.5% – 97.8% и нешто пониска специфичност од 73.3% - 92.9%. Употребата на современите алатки за визуелизација на ткивните аберации, бележат сензитивност од околу 30% и специфичност од 63%.

ЗАКЛУЧОК: Оралната цитологија, патохистолошкиот наод, молекуларните биолошки испитувања, како и примената на софистицирани визуелни средства во детекција на суспектните орални лезии, се смета дека ќе доведат до рано дијагностицирање и ќе го направи толкувањето на наодите далеку посигурно отколку порано.


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Николовски Б, Поповиќ-Моневска Д, Миновска А, Поповска М, Радојкова-Николовска В. Техники и методи за детекција и дијагноза на орални премалигни и малигни лезии. Maced Med Electr J [Internet]. 2015 Nov. 10 [cited 2023 Sep. 29];1(1):1-8. Available from: https://www.id-press.eu/mmej/article/view/419


Onofre MA, Sposto MR, Navarro CM. Reliability of toluidine blue application in the detection of oral epithelial dysplasia and in situ and invasive squamous cell carcinomas. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001;91(5):535-540. http://dx.doi.org/10.1067/moe.2001.112949 PMid:11346731

Epstein JB, Zhang L, Poh C, Nakamura H, Berean K, Rosin M. Increased allelic loss in toluidine blue–positive oral premalignant lesions. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003;95(1):45-50. http://dx.doi.org/10.1067/moe.2003.97 PMid:12539026

Epstein JB, Feldman R, Dolor RJ, Porter SR. The utility of tolonium chloride rinse in the diagnosis of recurrent or second primarycancers in patients with prior upper aerodigestive tract cancer. Head Neck. 2003;25(11):911-921. http://dx.doi.org/10.1002/hed.10309 PMid:14603451

Zhang L, Williams M, Poh CF, et al. Toluidine blue staining identifies high-risk primary oral premalignant lesions with poor outcome. Cancer Res. 2005;65(17):8017-8021. PMid:16140975

Ram S, Siar CH. Chemiluminescence as a diagnostic aid in the detection of oral cancer and potentially malignant epithelial lesions. Int J Oral Maxillofac Surg. 2005;34(5):521-527. http://dx.doi.org/10.1016/j.ijom.2004.10.008 PMid:16053872

Farah CS, McCullough MJ. A pilot case control study on the efficacy of acetic acid wash and chemiluminescent illumination (ViziLite) in the visualisation of oral mucosal white lesions. Oral Oncol. 2007;43(8):820-824. http://dx.doi.org/10.1016/j.oraloncology.2006.10.005 PMid:17169603

Lane PM, Gilhuly T, Whitehead P, et al. Simple device for the direct visualization of oral-cavity tissue fluorescence. J Biomed Opt. 2006;11(2):024006. http://dx.doi.org/10.1117/1.2193157 PMid:16674196

Poh CF, Zhang L, Anderson DW, et al. Fluorescence visualization detection of field alterations in tumor margins of oral cancer patients.Clin Cancer Res. 2006;12(22):6716-6722. http://dx.doi.org/10.1158/1078-0432.CCR-06-1317 PMid:17121891

Epstein JB, Silverman S Jr, Epstein JD, Lonky SA, Bride MA. Analysis of oral lesion biopsies identified and evaluated by visual examination, chemiluminescence and toluidine blue. Oral Oncol. 2008;44(6):538-544.

http://dx.doi.org/10.1016/j.oraloncology.2007.08.011 PMid:17996486

Poate TW, Buchanan JA, Hodgson TA, et al. An audit of the effi efficacy of the oral brush biopsy technique in a specialist oral medicine unit. Oral Oncol. 2004;40(8):829-834. http://dx.doi.org/10.1016/j.oraloncology.2004.02.005 PMid:15288839

Scheifele C, Schmidt-Westhausen AM, Dietrich T, Reichart PA. The sensitivity and specificity of the OralCDx technique: evaluation of103 cases. Oral Oncol. 2004;40(8):824-828. http://dx.doi.org/10.1016/j.oraloncology.2004.02.004 PMid:15288838

Driemel O, Kunkel M, Hullmann M, et al. Diagnosis of oral squamous cell carcinoma and its precursor lesions (in English and German). J Dtsch Dermatol Ges. 2007;5(12):1095-1100. http://dx.doi.org/10.1111/j.1610-0387.2007.06397.x PMid:18042091

Fischer DJ, Epstein JB, Morton TH Jr, Schwartz SM. Reliability of histologic diagnosis of clinically normal intraoral tissue adjacent to clinically suspicious lesions in former upper aerodigestive tract cancer patients. Oral Oncol. 2005;41(5):489-496. http://dx.doi.org/10.1016/j.oraloncology.2004.12.007 PMid:15878753

Fischer DJ, Epstein JB, Morton TH, Schwartz SM. Interobserver reliability in the histopathologic diagnosis of oral pre-malignant and malignant lesions. J Oral Pathol Med. 2004;33(2):65-70. http://dx.doi.org/10.1111/j.1600-0714.2004.0037n.x PMid:14720191

Thomson PJ. Field change and oral cancer: new evidence for widespread carcinogenesis? Int J Oral Maxillofac Surg. 2002;31(3): 262-266. http://dx.doi.org/10.1054/ijom.2002.0220 PMid:12190131

Dabelsteen E. ABO blood group antigens in oral mucosa. What is new? J Oral Pathol Med. 2002:31:65–70. http://dx.doi.org/10.1046/j.0904-2512.2001.00004.x PMid:11896825

Paterson IC, Eveson JW, Prime SS. Molecular changes in oral cancer may reflect aetiology and ethnic origin. Eur J Cancer B Oral Oncol. 1996;32(B):150–153.

Kaplan I, Vered M, Moskona D, Buchner A, Dayan D. An immunohistochemical study of p53 and PCNA in inflammatory papillary hyperplasia of the palate: a dilemma of interpretation. Oral Dis. 1998;4:194–199. http://dx.doi.org/10.1111/j.1601-0825.1998.tb00278.x PMid:9972170

De Paula AM, Carvalhais JN, Domingues MG, Barreto DC, Mesquita RA. Cell proliferation markers in the odontogenic keratocyst: effect of inflammation. J Oral Pathol Med. 2000;29:477–482. http://dx.doi.org/10.1034/j.1600-0714.2000.291001.x PMid:11048963

Bloor BK, Seddon SV, Morgan PR. Gene expression of differentiation-specific keratins in oral epithelial dysplasia and squamous cell carcinoma. Oral Oncol. 2001;37:251–261. http://dx.doi.org/10.1016/S1368-8375(00)00094-4

Schimming R, Hlawitschka M, Haroske G, Eckelt U. Prognostic relevance of DNA image cytometry in oral cavity carcinomas. Anal Quant Cytol Histol. 1998;20:43–51. PMid:9513690

Bundgaard T, Sorensen FB, Gaihede M, Sí¸gaard H, Overgaard J. Stereologic, histopathologic, flow cytometric, and clinical parameters in the prognostic evaluation of 74 patients with intraoral squamous cell carcinomas. Cancer. 1992;70:1–13. http://dx.doi.org/10.1002/1097-0142(19920701)70:1<1::AID-CNCR2820700102>3.0.CO;2-S

Saito T, Yamashita T, Notani K, Fukuda H, Mizuno S, Shindoh M, et al. Flow cytometric analysis of nuclear DNA content in oral leukoplakia: relation to clinicopathologic findings. Int J Oral Maxillofac Surg. 1995;24:44–47. http://dx.doi.org/10.1016/S0901-5027(05)80855-0

Hí¶gmo A, Munck-Wikland E, Kuylenstierna R, Lindholm J, Auer G. Nuclear DNA content and p53 immunostaining in metachronous preneoplastic lesions and subsequent carcinomas of the oral cavity. Head Neck. 1996;18:433–440. http://dx.doi.org/10.1002/(SICI)1097-0347(199609/10)18:5<433::AID-HED6>3.0.CO;2-6

Renan MJ. How many mutations are required for tumorigenesis? Implications from human cancer data. Mol Carcinog. 1993;7:139–146. http://dx.doi.org/10.1002/mc.2940070303 PMid:8489711

Rosin MP, Cheng X, Poh C, Lam WL, Huang Y, Lovas J, et al. Use of allelic loss to predict malignant risk for low-grade oral epithelial dysplasia. Clin Cancer Res. 2000;6:357–362. PMid:10690511

Lee JJ, Hong WK, Hittelman WN, Mao L, Lotan R, Shin DM, et al. Predicting cancer development in oral leukoplakia: ten years of translational research.Clin Cancer Res. 2000;6:1702–1710. PMid:10815888

Poh CF, Zhang L, Lam WL, Zhang X, An D, Chau C, et al. A high frequency of allelic loss in oral verrucous lesions may explain malignant risk. Lab Invest. 2001;81:629–634. http://dx.doi.org/10.1038/labinvest.3780271 PMid:11304582

Greenblatt MS, Bennett WP, Hollstein M, Harris CC. Mutations in the p53 tumor suppressor gene: clues to cancer etiology and molecular pathogenesis. Cancer Res. 1994;54:4855–4878. PMid:8069852

Nylander K, Dabelsteen E, Hall PA. The p53 molecule and its prognostic role in squamous cell carcinomas of the head and neck. J Oral Pathol Med. 2000;29:413–425. http://dx.doi.org/10.1034/j.1600-0714.2000.290901.x PMid:11016683

Califano J, van der Riet P, Westra W, Nawroz H, Clayman G, Piantadosi S, et al. Genetic progression model for head and neck cancer: implications for field cancerization. Cancer Res. 1996;56:2488–2492. http://dx.doi.org/10.1016/s0194-5998(96)80594-8

Shahnavaz SA, Regezi JA, Bradley G, Dube ID, Jordan RC. p53 gene mutations in sequential oral epithelial dysplasias and squamous cell carcinomas. J Pathol. 2000;190:417–422. http://dx.doi.org/10.1002/(SICI)1096-9896(200003)190:4<417::AID-PATH544>3.0.CO;2-G

Cruz IB, Snijders PJ, Meijer CJ, Braakhuis BJ, Snow GB, Walboomers JM, et al. p53 expression above the basal cell layer in oral mucosa is an early event of malignant transformation and has predictive value for developing oral squamous cell carcinoma. J Pathol. 1998;184:360–368. http://dx.doi.org/10.1002/(SICI)1096-9896(199804)184:4<360::AID-PATH1263>3.0.CO;2-H

Chang K-W, Lin S-C, Kwan P-C, Wong Y-K. Association of aberrant p53 and p21WAF1 immunoreactivity with the outcome of oral verrucous leukoplakia in Taiwan. J Oral Pathol Med. 2000;29:56–62. http://dx.doi.org/10.1034/j.1600-0714.2000.290202.x

Warnakulasuriya S. Cancers of the oral cavity and pharynx. In: Genetic basis of human cancer. Vogelstein B, Kinzler KW, editors. London: McGraw-Hill, 2002:pp. 773-784.

Piffko J, Bankfalvi A, Joos U, í–fner D, Krassort M, Schmid KW. Immunophenotypic analysis of normal mucosa and squamous cell carcinoma of the oral cavity. Cancer Detect Prev. 1999;23:45–56. http://dx.doi.org/10.1046/j.1525-1500.1999.09903.x PMid:9892990

Ravn V, Dabelsteen E. Tissue distribution of histo-blood group antigens. APMIS. 2000;108:1–28. http://dx.doi.org/10.1034/j.1600-0463.2000.d01-1.x PMid:10698081

Su L, Morgan PR, Lane EB. Keratin 14 and 19 expression in normal, dysplastic and malignant oral epithelia. A study using in situ hybridization and immunohistochemistry. J Oral Pathol Med. 1996;25:293–301. http://dx.doi.org/10.1111/j.1600-0714.1996.tb00265.x PMid:8887072