Open Access Macedonian Journal of Medical Sciences Open Access Macedonian Journal of Medical Sciences (OAMJMS) is an international peer reviewed journal published four times per year by the ID Design 2012/DOOEL Skopje on behalf of Foundation Macedonian Donor Registry (MKDR), Skopje Scientific Foundation SPIROSKI, Skopje, Republic of Macedonia en-US Open Access Macedonian Journal of Medical Sciences 1857-9655 <p>All rights reserved.</p> Identification of Active Compounds of Ethanol Extract of Citrus amblycarpa leaves by Analysis of Thin-layer Chromatography and Gas Chromatography-Mass Spectrometry as Bioinsecticide Candidates for Mosquitoes <p><strong>BACKGROUND: </strong>The use of active compounds from plants becomes an alternative to control mosquitoes nowadays and in the future because they are environmentally-friendly and do not cause health problems. <em>Citrus amblycarpa </em>is a local orange of South Kalimantan potential as bioinsecticidal, which commonly used for controlling mosquitoes. Therefore, research needs to be done to find out the benefits of <em>C. amblycarpa </em>leaves as bioinsecticidal.</p> <p><strong>AIM: </strong>The research aimed to identify active compounds contained in the extract ethanol of <em>C. amblycarpa </em>leaves as bioinsecticidal against mosquitoes.</p> <p><strong>RESULTS: </strong>Based on thin-layer chromatography test, there were some secondary metabolite compounds found such as terpenoids/steroids, flavonoids, polyphenols, and saponins. Gas chromatography-mass spectrometry (GC-MS) test revealed that there were ten primary components of the fraction. The components were Maragenin I (18,82%), 1,3-benzenedicarboxamide (12.28%), 2,3,8-trioxocephalotaxane (10.39%), aristolone, 2H-cyclopropa[a] naphthalene-2-one, noruns-12-ene (7.46%), palmitic acid, n-hexadecanoic acid (7.21%), stigmasterol, demecolcine (7.03%), alpha-tocopherol (5.88%), 2,4,5-trimethylphenol, pseudocumenol (4.21%), germacrene-D (3.45%), and 9-octadecenoic acid (3.36%).</p> <p><strong>CONCLUSION: </strong>These active compounds possess biological activity as bioinsecticidal. It was expected that those active compounds in <em>C. amblycarpa </em>leaves could be applied for controlling mosquitoes by replacing the use of resistant temephos.</p> Kasman Kasman Nuning Irnawulan Ishak Poedji Hastutiek Endang Suprihati Anwar Mallongi Copyright (c) 2020 Kasman Kasman, Nuning Irnawulan Ishak, Poedji Hastutiek, Endang Suprihati, Anwar Mallongi (Author) 2020-09-20 2020-09-20 8 T2 1 6 10.3889/oamjms.2020.5207