Open Access Macedonian Journal of Medical Sciences https://www.id-press.eu/mjms Open Access Macedonian Journal of Medical Sciences (OAMJMS) is an international peer reviewed journal published four times per year by the ID Design 2012/DOOEL Skopje on behalf of Foundation Macedonian Donor Registry (MKDR), Skopje Scientific Foundation SPIROSKI, Skopje, Republic of Macedonia en-US Open Access Macedonian Journal of Medical Sciences 1857-9655 <p>All rights reserved.</p> Identification of Active Compounds of Ethanol Extract of Citrus amblycarpa leaves by Analysis of Thin-layer Chromatography and Gas Chromatography-Mass Spectrometry as Bioinsecticide Candidates for Mosquitoes https://www.id-press.eu/mjms/article/view/5207 <p><strong>BACKGROUND: </strong>The use of active compounds from plants becomes an alternative to control mosquitoes nowadays and in the future because they are environmentally-friendly and do not cause health problems. <em>Citrus amblycarpa </em>is a local orange of South Kalimantan potential as bioinsecticidal, which commonly used for controlling mosquitoes. Therefore, research needs to be done to find out the benefits of <em>C. amblycarpa </em>leaves as bioinsecticidal.</p> <p><strong>AIM: </strong>The research aimed to identify active compounds contained in the extract ethanol of <em>C. amblycarpa </em>leaves as bioinsecticidal against mosquitoes.</p> <p><strong>RESULTS: </strong>Based on thin-layer chromatography test, there were some secondary metabolite compounds found such as terpenoids/steroids, flavonoids, polyphenols, and saponins. Gas chromatography-mass spectrometry (GC-MS) test revealed that there were ten primary components of the fraction. The components were Maragenin I (18,82%), 1,3-benzenedicarboxamide (12.28%), 2,3,8-trioxocephalotaxane (10.39%), aristolone, 2H-cyclopropa[a] naphthalene-2-one, noruns-12-ene (7.46%), palmitic acid, n-hexadecanoic acid (7.21%), stigmasterol, demecolcine (7.03%), alpha-tocopherol (5.88%), 2,4,5-trimethylphenol, pseudocumenol (4.21%), germacrene-D (3.45%), and 9-octadecenoic acid (3.36%).</p> <p><strong>CONCLUSION: </strong>These active compounds possess biological activity as bioinsecticidal. It was expected that those active compounds in <em>C. amblycarpa </em>leaves could be applied for controlling mosquitoes by replacing the use of resistant temephos.</p> Kasman Kasman Nuning Irnawulan Ishak Poedji Hastutiek Endang Suprihati Anwar Mallongi Copyright (c) 2020 Kasman Kasman, Nuning Irnawulan Ishak, Poedji Hastutiek, Endang Suprihati, Anwar Mallongi (Author) http://creativecommons.org/licenses/by-nc/4.0 2020-09-20 2020-09-20 8 T2 1 6 10.3889/oamjms.2020.5207