Hypoxia-preconditioned MSCs Have Superior Effect in Ameliorating Renal Function on Acute Renal Failure Animal Model

  • Agung Putra Stem Cell and Cancer Research (SCCR) Laboratory, Medical Faculty, UNISSULA, Semarang, Indonesia; Department of Postgraduate Biomedical Science, Medical Faculty, UNISSULA, Semarang, Indonesia; Departement of Pathological Anatomy of Medical Faculty, UNISSULA, Semarang, Indonesia http://orcid.org/0000-0003-4261-9437
  • Dannis Pertiwi Department of Clinical Pathology, UNISSULA, Semarang, Indonesia
  • Meidona Nurul Milla Department of Anatomy, Medical Faculty, UNISSULA, Semarang, Indonesia
  • Ulfah Dian Indrayani Department of Histology, Medical Faculty, UNISSULA, Semarang, Indonesia
  • Durotul Jannah Department of Neurology, Medical Faculty, UNISSULA, Semarang, Indonesia
  • Menik Sahariyani Department of Parasitology, UNISSULA, Semarang, Indonesia
  • Setyo Trisnadi Department of Postgraduate Biomedical Science, Medical Faculty, UNISSULA, Semarang, Indonesia
  • Joko Wahyu Wibowo Department of Postgraduate Biomedical Science, Medical Faculty, UNISSULA, Semarang, Indonesia
Keywords: HP-MSCs, N-MSCs, ARF, BUN, Creatinin


BACKGROUND: Acute renal failure (ARF) is a serious disease characterised by a rapid loss of renal functions due to nephrotoxic drug or ischemic insult. The clinical treatment approach such as dialysis techniques and continuous renal enhancement have grown rapidly during past decades. However, there is yet no significant effect in improving renal function. Hypoxia-preconditioned mesenchymal stem cells (HP-MSCs) have positive effects on the in vitro survival and stemness, in addition to angiogenic potential.

AIM: In this study, we aimed to analyse the effect of HP-MSCs administration in improving renal function, characterised by blood urea nitrogen (BUN) and creatinine level.

METHODS: A group of 15 male Wistar rats weighing 250 g to 300 g were used in this study (n = 5 for each group). Rats were randomly distributed into 3 groups: Vehicle control (Veh) as a control group, HP-MSCs and normoxia MSCs (N-MSCs) as the treatment group. Renal function was evaluated based on the BUN and creatinine levels using the colourimetric method on day 5 and 13. The histological analysis using HE staining was performed on day 13.

RESULTS: The result showed there is a significant decrease in BUN and creatinine level (p < 0.05). The histological analysis of renal tissue also showed a significant decrease between Veh and treatment group (p < 0.05).

CONCLUSION: Based on this study, we conclude that HP-MSCs have a superior beneficial effect than N-MSCs in improving renal function in an animal model of gentamicin-induced ARF.


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How to Cite
Putra A, Pertiwi D, Milla MN, Indrayani UD, Jannah D, Sahariyani M, Trisnadi S, Wibowo JW. Hypoxia-preconditioned MSCs Have Superior Effect in Ameliorating Renal Function on Acute Renal Failure Animal Model. Open Access Maced J Med Sci [Internet]. 2019Jan.30 [cited 2021Jan.24];7(3):305-10. Available from: https://www.id-press.eu/mjms/article/view/oamjms.2019.049
A - Basic Science