Male Infertility and Sperm DNA Fragmentation

  • Afrim Zeqiraj Department of Biochemistry, FAMA Colleague, Prishtina, Kosovo
  • Sheqibe Beadini Department of Biology, University of Tetovo
  • Nexhbedin Beadini Department of Biology, University of Tetovo
  • Hesat Aliu Department of Biology, University of Tetovo
  • Zafer Gashi Department of Biochemistry, FAMA Colleague, Prishtina, Kosovo
  • Shkelzen Elezaj Clinic of Urology, Regional Hospital of Peja, Kosovo
  • Sadi Bexheti Faculty of Medicine, University of Tetovo
  • Agim Shabani Department of Chemistry, University of Tetovo
Keywords: Sperm DNA fragmentation, (SCD), IVF/ICSI


BACKGROUND: One of the main factors affecting male infertility is DNA fragmentation in sperm. Male infertility is a heterogeneous group of disorders, known causes account for only 30-50%, and unknown cause (idiopathic) constitute the rest. Infertility involves nearly 15% of couples in the reproductive age, and only the male problem involves about 40% of the problems.

AIM: We have studied our DNA damage to sperm cells of a group of infertile males (113 patients) with abnormal sperm parameters (oligoasthenospermia and oligospermia) and a group of male patients (80 patients) with normal semen parameters (normospermia) to document whether the Sperm Chromatin Dispersion (SCD) analysis could increase the information obtained from the sperm routine analysis to explain the causes of infertility.

MATERIALS: A group of 193 patients were analysed, 113 patients in the working group and 80 patients in the control group were screened. The ejaculate samples were taken by the patient to whom the reason for the analysis was explained. All patients were from the Republic of Kosovo. Samples are collected from 2014/2018. Sperm Chromatin Dispersion (SCD) analyses in the ejaculate were analysed by the Biolab Zafi laboratory in Peja.

RESULTS: Clinical data were compared between the two groups by one-way ANOVA, mean ± SD, student's t-test. A p-value of less than P < 0.05% was considered statistically significant. Outcomes: In our study, we have gained significant (P < 0.05) results in the workgroup and the control group across all hormonal parameters, sperm parameters, and fragmented DNA in the sperm.

CONCLUSION: Based on our obtained results we can conclude that DNA fragmentation in spermatozoa is useful in the selection of unsuitable DNA sperm for use in ART methods. We conclude that our DNA fragmentation analysis results are encouraging and can be used for diagnostic purposes in determining male infertility.


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How to Cite
Zeqiraj A, Beadini S, Beadini N, Aliu H, Gashi Z, Elezaj S, Bexheti S, Shabani A. Male Infertility and Sperm DNA Fragmentation. Open Access Maced J Med Sci [Internet]. 2018Aug.14 [cited 2020Nov.29];6(8):1342-5. Available from:
A - Basic Science