Male Infertility and Sperm DNA Fragmentation

  • Afrim Zeqiraj Department of Biochemistry, FAMA Colleague, Prishtina, Kosovo http://orcid.org/0000-0001-8145-4503
  • Sheqibe Beadini Department of Biology, University of Tetovo
  • Nexhbedin Beadini Department of Biology, University of Tetovo
  • Hesat Aliu Department of Biology, University of Tetovo
  • Zafer Gashi Department of Biochemistry, FAMA Colleague, Prishtina, Kosovo
  • Shkelzen Elezaj Clinic of Urology, Regional Hospital of Peja, Kosovo
  • Sadi Bexheti Faculty of Medicine, University of Tetovo
  • Agim Shabani Department of Chemistry, University of Tetovo
Keywords: Sperm DNA fragmentation, (SCD), IVF/ICSI

Abstract

BACKGROUND: One of the main factors affecting male infertility is DNA fragmentation in sperm. Male infertility is a heterogeneous group of disorders, known causes account for only 30-50%, and unknown cause (idiopathic) constitute the rest. Infertility involves nearly 15% of couples in the reproductive age, and only the male problem involves about 40% of the problems.

AIM: We have studied our DNA damage to sperm cells of a group of infertile males (113 patients) with abnormal sperm parameters (oligoasthenospermia and oligospermia) and a group of male patients (80 patients) with normal semen parameters (normospermia) to document whether the Sperm Chromatin Dispersion (SCD) analysis could increase the information obtained from the sperm routine analysis to explain the causes of infertility.

MATERIALS: A group of 193 patients were analysed, 113 patients in the working group and 80 patients in the control group were screened. The ejaculate samples were taken by the patient to whom the reason for the analysis was explained. All patients were from the Republic of Kosovo. Samples are collected from 2014/2018. Sperm Chromatin Dispersion (SCD) analyses in the ejaculate were analysed by the Biolab Zafi laboratory in Peja.

RESULTS: Clinical data were compared between the two groups by one-way ANOVA, mean ± SD, student's t-test. A p-value of less than P < 0.05% was considered statistically significant. Outcomes: In our study, we have gained significant (P < 0.05) results in the workgroup and the control group across all hormonal parameters, sperm parameters, and fragmented DNA in the sperm.

CONCLUSION: Based on our obtained results we can conclude that DNA fragmentation in spermatozoa is useful in the selection of unsuitable DNA sperm for use in ART methods. We conclude that our DNA fragmentation analysis results are encouraging and can be used for diagnostic purposes in determining male infertility.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

Practice Committee of the American Society for Reproductive Medicine. Definitions of infertility and recurrent pregnancy loss: a committee opinion. Fertility and sterility. 2013; 99(1):63. https://doi.org/10.1016/j.fertnstert.2012.09.023 PMid:23095139

Jungwirth A, Diemer T, Dohle GR, Giwercman A, Kopa Z, Krausz C, Tournaye H. Male Infertility. European Association of Urology. 2015; 62(2):324-32. https://doi.org/10.1016/j.eururo.2012.04.048 PMid:22591628

O'Flynn O'Brien KL, Varghese AC, Agarwal A. The genetic causes of male factor infertility. Fertil Steril. 2010; 93(1):1-12. https://doi.org/10.1016/j.fertnstert.2009.10.045 PMid:20103481

Marchettini P, Solaro C, Sommer C, Cruccu G. To become a pain specialist one has to understand the nervous system, yet the specialists of the nervous system still have a long way to go before understanding pain. Neurological Sciences. 2007; 28(4):161-162. https://doi.org/10.1007/s10072-007-0812-2 PMid:17690843

Martin FH. Hormones of reproductive system In: Fundamental of anatomy and physiology. 2001; 1057.

Guyton AC. Test book of medical physiology. W.B. Saunders and Company, Philadelphia, U.S.A., 1981:972-1004.

Amelar RD. Infertility in man. FA Davis Company, Philadelphia, U.S.A., 1966:30-53.

Merino G, Carranza-Lira S. Semen characteristics, endocrine profiles, and testicular biopsies of infertile men of different ages. Archives of andrology. 1995; 35(3):219-24. https://doi.org/10.3109/01485019508987874 PMid:8585777

Høst E, Ernst E, Lindenberg S, Smidt-Jensen S. Morphology of spermatozoa used in IVF and ICSI from oligozoospermic men. Reproductive biomedicine online. 2001; 3(3):212-5. https://doi.org/10.1016/S1472-6483(10)62038-X

Agarwal A, Said TM. Role of sperm chromatin abnormalities and DNA damage in male infertility. Human reproduction update. 2003; 9(4):331-45. https://doi.org/10.1093/humupd/dmg027 PMid:12926527

Gandini L, Lombardo F, Paoli D, Caruso F, Eleuteri P, Leter G, Ciriminna R, Culasso F, Dondero F, Lenzi A, Spano M. Fullâ€term pregnancies achieved with ICSI despite high levels of sperm chromatin damage. Human reproduction. 2004; 19(6):1409-17. https://doi.org/10.1093/humrep/deh233 PMid:15117904

Li TC. Recurrent miscarriage: principles of management. Human reproduction (Oxford, England). 1998; 13(2):478-82. https://doi.org/10.1093/humrep/13.2.478

Bungum M, Humaidan P, Axmon A, Spano M, Bungum L, Erenpreiss J, Giwercman A. Sperm DNA integrity assessment in prediction of assisted reproduction technology outcome. Human reproduction. 2006; 22(1):174-9. https://doi.org/10.1093/humrep/del326 PMid:16921163

Fernández JL, Muriel L, Goyanes V, Segrelles E, Gosálvez J, Enciso M, LaFromboise M, De Jonge C. Simple determination of human sperm DNA fragmentation with an improved sperm chromatin dispersion test. Fertility and sterility. 2005; 84(4):833-42. https://doi.org/10.1016/j.fertnstert.2004.11.089 PMid:16213830

Yılmaz S, Zergeroğlu AD, Yılmaz E, Sofuoglu K, Delikara N, Kutlu P. Effects of sperm DNA fragmentation on semen parameters and ICSI outcome determined by an improved SCD test, Halosperm. Int J Fertil Steril. 2010; 4(2).

Virro MR, Larson-Cook KL, Evenson DP. Sperm chromatin structure assay (SCSA®) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles. Fertility and sterility. 2004; 81(5):1289-95. https://doi.org/10.1016/j.fertnstert.2003.09.063 PMid:15136092

World Health Organisation. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Cambridge university press, 1999.

Subhan F, Tahir F, Ahmad R, Khan ZU. The study of azoospermic patients in relation to their hormonal profile [LH, FSH and Testosterone]. Rawal Med J. 1995; 22:25-27.

Sheikh MA, Khan MS, Danyal A, Zaidi SS, Begum B, Turabi A. Azoospermia and Oligozoospermia: Semen and Hormonal analysis of patients. Professional Med J. 2005; 12:80-4.

De-Krester DM (1979) Endocrinology of male infertility. Br Med Bull. 1979; 35:187-192. https://doi.org/10.1093/oxfordjournals.bmb.a071568

Nistal M, Jimenez F, Paniagua R. Sertoli-cell types in sertoli cell only morphology and aetiology. Histopathology. 1990; 16: 173-180. https://doi.org/10.1111/j.1365-2559.1990.tb01086.x PMid:2182507

Turek PJ, Kim M, Gilbaugh JH, Lipshultz LI. The clinical characteristics of 82 patients with Sertoli cell-only testis histology. Fertility and sterility. 1995; 64(6):1197-200. https://doi.org/10.1016/S0015-0282(16)57984-9

Reyesâ€Fuentes A, Chavarria ME, Aguilera G, Rosado A, Iranmanesh A, Veldhuis JD. Deconvolution analysis of bioassayable LH secretion and halfâ€life in men with idiopathic oligoasthenospermia. International journal of andrology. 1997; 20(2):118-25. https://doi.org/10.1046/j.1365-2605.1997.00104.x PMid:9292323

Meeker JD, Godfreyâ€Bailey L, Hauser R. Relationships between serum hormone levels and semen quality among men from an infertility clinic. Journal of andrology. 2007; 28(3):397-406. https://doi.org/10.2164/jandrol.106.001545 PMid:17135633

Sergerie M, Laforest G, Bujan L, Bissonnette F, Bleau G. Sperm DNA fragmentation: threshold value in male fertility. Human reproduction. 2005; 20(12):3446-51. https://doi.org/10.1093/humrep/dei231 PMid:16085665

Diallo MS, Faye O, Diallo AS, Diallo Y, Diao B. Increased DNA fragmentation in patients with infertility in Dakar (Senegal). Advances in Reproductive Sciences. 2015; 3(04):97. https://doi.org/10.4236/arsci.2015.34012

Published
2018-08-14
How to Cite
1.
Zeqiraj A, Beadini S, Beadini N, Aliu H, Gashi Z, Elezaj S, Bexheti S, Shabani A. Male Infertility and Sperm DNA Fragmentation. Open Access Maced J Med Sci [Internet]. 2018Aug.14 [cited 2020Nov.29];6(8):1342-5. Available from: https://www.id-press.eu/mjms/article/view/oamjms.2018.311
Section
A - Basic Science