Thr83ala Gene Polymorphism Association with Arterial Calcification, Acute Coronary Syndrome and Ischemic Strokes in Older Adults

  • Yurii O. Ataman Family Medicine Department, Sumy State University, Sumy
  • Albina V. Zharkova Family Medicine Department, Sumy State University, Sumy
  • Andrii V. Grek Internal Medicine Department, Sumy State University, Sumy
  • Tetiana M. Rudenko Family Medicine Department, Sumy State University, Sumy
  • Maryna A. Gordina Family Medicine Department, Sumy State University, Sumy
Keywords: Lower extremity arterial calcification, Matrix Gla protein, Gene polymorphism

Abstract

BACKGROUND: Calcification of the arteries of the lower extremities is a very common pathological process, which has an independent significance in the development of cardiovascular diseases. There is evidence that development of calcification of the arteries correlates with a mutation of the MGP protein gene representing by the Thr83Ala polymorphism.

AIM: The purpose of the study was to analyse the connection of Thr83Ala polymorphism of the MGP gene with the development of calcification of the arteries of the lower extremities.

METHODS: The study involved 80 patients. Half of them had signs of calcification of the arteries of the lower extremities. The allelic Thr83Ala polymorphism of the MGP protein gene was determined by polymerase chain reaction, establishing the presence of calcification of the arteries by radiological and dopplerographic methods.

RESULTS: The study aimed to analyse the association of the Thr83Ala polymorphism of the matrix Gla protein gene with the development of calcification of the arteries of the lower extremities. The data obtained suggest that the replacement of threonine by alanine in the 83rd position of the MGP molecule can affect the functional properties of the protein and in particular its anticarcinogenic properties. Although there was no difference in the distribution of different variants of the genotype by Thr83Ala to the MGP gene polymorphism in patients with CA and healthy patients, but in the distribution of genotypes in the comparison groups separated by sex, it was found that in women, carriage of the Ala allele in a homozygous state is a factor, which protects the development of arterial calcination in the elderly and senile.

CONCLUSION: Differences in the distribution of different variants of the genotype according to Thr83Ala to the polymorphism of the MGP gene between patients with CAD and healthy patients do not exceed the limits of statistical significance. In the distribution of genotypes in the comparison groups divided by sex, it was found that in women the carrier of the Ala-allele in the homozygous state is a factor that prevents the development of Menkeberg arteriosclerosis in the elderly and old age.

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References

Ataman YA, Grek AV, Opolonskaya NA. The study of calcification of the vessels of the lower limbs in elderly and senile patients. Actual problems of modern medicine. 2011; 4(36):8-12.

Garbuzova VY, Guryanova VL, Parchomanko OM, et al. Frequency of allelic variants of the gene of matrix Gla-protein (MGP) in patients with the acute coronary syndrome. Physiological Journal. 2011; 57(3):16-24.

Yena LM, Artemenko VO, Chayalo PP, et al. Arterial stiffness and vascular ageing Practical angiology. 2007; 2:14-15.

Talayeva TV, Bratus VV. Vascular calcification: reality and hypotheses. Health of Ukraine. 2014; 11(2):56–60.

Price PA, Faus SA, Williamson MK. Warfarin-induced artery calcification is accelerated by growth and vitamin D. Arteriosclerosis, thrombosis, and vascular biology. 2000 20(2):317-27. https://doi.org/10.1161/01.ATV.20.2.317 PMid:10669626

Cancela L, Hsieh CL, Francke U, Price PA. Molecular structure, chromosome assignment, and promoter organization of the human matrix Gla protein gene. Journal of Biological Chemistry. 1990; 265(25):15040-8. PMid:2394711

Hale JE, Williamson MK, Price PA. Carboxyl-terminal proteolytic processing of matrix Gla protein. J Biol Chem. 1991; 266:21145-21149. PMid:1939157

Rice JS, Williamson MK, Price PA. Isolation and sequence of the vitamin Kâ€dependent matrix Gla protein from the calcified cartilage of the soupfin shark. Journal of Bone and Mineral Research. 1994; 9(4):567-76. https://doi.org/10.1002/jbmr.5650090417 PMid:8030445

Price PA, Rice JS, Williamson MK. Conserved phosphorylation of serines in the Ser-X-Glu/Ser(P) sequences of the vitamin K-dependent matrix Gla protein from shark, lamb, rat, cow, and human. Protein Sci. 1994; 3:822- 830. https://doi.org/10.1002/pro.5560030511 PMid:8061611 PMCid:PMC2142713

Doherty TM, Fitzpatrick LA, Inoue D, Qiao JH, Fishbein MC, Detrano RC, Shah PK, Rajavashisth TB. Molecular, endocrine, and genetic mechanisms of arterial calcification. Endocrine reviews. 2004; 25(4):629-72. https://doi.org/10.1210/er.2003-0015 PMid:15294885

Guzman RJ. Clinical, cellular, and molecular aspects of arterial calcification. Journal of vascular surgery. 2007; 45(6):A57-63. https://doi.org/10.1016/j.jvs.2007.02.049 PMid:17544025 PMCid:PMC2435088

Raggi P, Bellasi A. Clinical assessment of vascular calcification. Adv Chronic Kidney Dis. 2007; 14(1):37-45. https://doi.org/10.1053/j.ackd.2006.10.006 PMid:17200042

Ataman AV, Garbusova VY, Ataman YA, Matlaj OI, Obuchova OA. Investigation of the MGP promoter and exon 4 polymorphisms in patients with ischemic stroke in the Ukrainian population. Journal of Cell & Molecular Biology. 2012; 10(1).

Luo G, Ducy P, McKee MD, Pinero GJ, Loyer E, Behringer RR, Karsenty G. Spontaneous calcification of arteries and cartilage in mice lacking matrix GLA protein. Nature. 1997; 386(6620):78. https://doi.org/10.1038/386078a0 PMid:9052783

Price PA, Faus SA, Williamson MK. Warfarin causes rapid calcification of the elastic lamellae in rat arteries and heart valves. Arteriosclerosis, thrombosis, and vascular biology. 1998; 18(9):1400-7. https://doi.org/10.1161/01.ATV.18.9.1400

Published
2018-08-15
How to Cite
1.
Ataman YO, Zharkova AV, Grek AV, Rudenko TM, Gordina MA. Thr83ala Gene Polymorphism Association with Arterial Calcification, Acute Coronary Syndrome and Ischemic Strokes in Older Adults. Open Access Maced J Med Sci [Internet]. 2018Aug.15 [cited 2020Nov.29];6(8):1365-9. Available from: https://www.id-press.eu/mjms/article/view/oamjms.2018.297
Section
B - Clinical Sciences