Detection of Biofilm Producing Staphylococci among Different Clinical Isolates and Its Relation to Methicillin Susceptibility

  • Rania M. Abdel Halim Clinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo
  • Nevine N. Kassem Clinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo
  • Basma S. Mahmoud Clinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo
Keywords: Biofilm, Staphylococci, Methicillin, TCP, TM, CRA

Abstract

AIMS: To evaluate three in vitro phenotypic methods; tissue culture plate, tube method, and Congo red agar for detection of biofilm formation in staphylococci and assess the relation of biofilm formation with methicillin resistance and anti-microbial resistance.

METHODS: The study included 150 staphylococcal isolates. Biofilm detection in staphylococci was performed using tissue culture plate, tube method, and Congo red agar.

RESULTS: Tissue culture plate, tube method, and Congo red agar detected 74%, 42.7%, and 1.3% biofilm producing staphylococci respectively. S. aureus isolates were more common biofilm producers (53.2%) than CONS (46.8%). Biofilm production in CONS species was highest in S. hemolyticus (57.7%). Tube method was 51.4% sensitive, 82.1% specific. As for Congo red agar, sensitivity was very low (0.9%), but specificity was 97.4%. Biofilm producers were mostly; isolated from blood specimens (82.6%) and detected in methicillin-resistant strains 96/111 (86.5%). They were resistant to most antibiotics except vancomycin and linezolid.

CONCLUSIONS: Tissue culture plate is a more quantitative and reliable method for detection of biofilm producing staphylococci compared to tube method and Congo red agar. Hence, it can still be used as a screening method for biofilm detection. Vancomycin and Linezolid are the most sensitive antibiotics among biofilm producing staphylococci.

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Published
2018-08-05
How to Cite
1.
Abdel Halim RM, Kassem NN, Mahmoud BS. Detection of Biofilm Producing Staphylococci among Different Clinical Isolates and Its Relation to Methicillin Susceptibility. Open Access Maced J Med Sci [Internet]. 2018Aug.5 [cited 2020Nov.26];6(8):1335-41. Available from: https://www.id-press.eu/mjms/article/view/oamjms.2018.246
Section
A - Basic Science