Utility of Tissue Inhibitor Metalloproteinase-1 and Osteopontin as Prospective Biomarkers of Early Cardiovascular Complications in Type 2 Diabetes

  • Doaa Samir Salah El-Din Chemistry, Faculty of Science, Cairo University, Giza 12613
  • Ahmed Ibrahim Amin Chemistry, Faculty of Science, Cairo University, Giza 12613
  • Ahmed Osman Egiza Chemistry, Faculty of Science, Cairo University, Giza 12613
Keywords: Molecular markers, expression level, tissue inhibitor metalloproteinase-1, OPN concentrations, diabetic cardiovascular diseases

Abstract

AIM: This work investigated associations between tissue inhibitor metalloproteinase-1 and diabetic cardiovascular diseases in type 2 diabetic patients; also it investigated the role of osteopontin in the diagnosis of type 2 cardiovascular diabetes complications.

SUBJECTS AND METHODS: These were examined on eighty subjects, divided into three groups as follows: twenty volunteer healthy control subjects, thirty type 2 diabetes mellitus (DM) patients, and thirty cardiovascular, diabetic patients. Full clinical measurements were carried out, and the expression level of tissue inhibitor metalloproteinase-1 in blood samples was analysed by real-time PCR, using gene-specific primer pairs. Also osteopontin concentrations had been measured by the enzyme-linked immunosorbent assay. Data were tested statistically by parametric tests.

RESULTS: The concentrations of osteopontin and the expression levels of tissue inhibitor metalloproteinase-1 were significantly increased in diabetic and cardiovascular diabetic groups compared to control group also they were significantly increased in the cardiovascular diabetic group compared to the diabetic group.

CONCLUSION: Tissue inhibitor metalloproteinase-1 and osteopontin concentrations were significantly increased in diabetic patients with cardiovascular complications than other groups.

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Published
2018-02-10
How to Cite
1.
Salah El-Din DS, Amin AI, Egiza AO. Utility of Tissue Inhibitor Metalloproteinase-1 and Osteopontin as Prospective Biomarkers of Early Cardiovascular Complications in Type 2 Diabetes. Open Access Maced J Med Sci [Internet]. 2018Feb.10 [cited 2020Nov.29];6(2):314-9. Available from: https://www.id-press.eu/mjms/article/view/oamjms.2018.081
Section
B - Clinical Sciences