Preclinical Assessment of the Proliferation Capacity of Gingival and Periodontal Ligament Stem Cells from Diabetic Patients

  • Mostafa Assem National Research Centre, Cairo
  • Samia Kamal Cairo University Faculty of Oral and Dental Medicine, Cairo
  • Dina Sabry Cairo University, Kasr Alainy Faculty of Medicine, Cairo
  • Nadia Soliman National Research Centre, Cairo
  • Riham M. Aly National Research Centre, Cairo
Keywords: Proliferation, Survivin, Gingival Stem cells, Periodontal Ligament Stem Cells, Diabetes

Abstract

BACKGROUND: Stem cells have recently received great interest as potential therapeutics alternative for a variety of diseases. The oral and maxillofacial region, in particular, encompasses a variety of distinctive mesenchymal (MSC) populations and is characterized by a potent multilineage differentiation capacity.

AIM: In this report, we aimed to investigate the effect of diabetes on the proliferation potential of stem cells isolated from controlled diabetic patients (type 2) and healthy individuals.

SUBJECTS & METHODS: The proliferation rate of gingival and periodontal derived stem cells isolated from diabetic & healthy individuals were compared using MTT Assay. Expression levels of Survivin in isolated stem cells from all groups were measured by qRt - PCR.

RESULTS: There was a significantly positive correlation between proliferation rate and expression of Survivin in all groups which sheds light on the importance of Survivin as a reliable indicator of proliferation. The expression of Survivin further confirmed the proliferation results from MTT Assay where the expression of stem cells from non - diabetic individuals was higher than diabetic patients. Conclusion: Taking together all the results, it could be concluded that PDLSC and GSC are promising candidates for autologous regenerative therapy due to their ease of accessibility in addition to their high proliferative rates.

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References

Zhang Q, Shi S, Liu Y, et al. Mesenchymal stem cells derived from human gingiva are capable of immunomodulatory functions and ameliorate inflammation-related tissue destruction in experimental colitis. J Immunol. 2009;183(12):7787-7798. https://doi.org/10.4049/jimmunol.0902318 PMid:19923445 PMCid:PMC2881945

Fournier BP, Ferre FC, Couty L, et al. Multipotent progenitor cells in gingival connective tissue. Tissue Eng Part A. 2010; 16(9):2891-2899. https://doi.org/10.1089/ten.tea.2009.0796 PMid:20412029

Mitrano TI, Grob MS, Carrion F, et al. Culture and characterization of mesenchymal stem cells from human gingival tissue. J Periodontol. 2010; 81(6):917-925. https://doi.org/10.1902/jop.2010.090566 PMid:20450355

Su WR, Zhang QZ, Shi SH, Nguyen AL, Le AD. Human gingiva-derived mesenchymal stromal cells attenuate contact hypersensitivity via prostaglandin E2-dependent mechanisms. Stem Cells. 2011; 29(11):1849-1860. https://doi.org/10.1002/stem.738 PMid:21987520

Tang L, Li N, Xie H, Jin Y. Characterization of mesenchymal stem cells from human normal and hyperplastic gingiva. J Cell Physiol. 2011; 226(3):832-842. https://doi.org/10.1002/jcp.22405 PMid:20857425

Hermann A, Liebau S, Gastl R, et al. Comparative analysis of neuroectodermal differentiation capacity of human bone marrow stromal cells using various conversion protocols. J Neurosci Res. 2006; 83(8):1502-1514. https://doi.org/10.1002/jnr.20840 PMid:16612831

Kuroda Y, Kitada M, Wakao S, et al. Unique multipotent cells in adult human mesenchymal cell populations. Proc Natl Acad Sci U S A. 2010; 107(19):8639-8643. https://doi.org/10.1073/pnas.0911647107 PMid:20421459 PMCid:PMC2889306

Tomar GB, Srivastava RK, Gupta N, et al. Human gingiva-derived mesenchymal stem cells are superior to bone marrow-derived mesenchymal stem cells for cell therapy in regenerative medicine. Biochem Biophys Res Commun. 2010; 393(3):377-383. https://doi.org/10.1016/j.bbrc.2010.01.126 PMid:20138833

Yamamoto N, Maeda H, Tomokiyo A, et al. Expression and effects of glial cell line- derived neurotrophic factor on periodontal ligament cells. J Clin Periodontol. 2012; 39(6):556-564. https://doi.org/10.1111/j.1600-051X.2012.01881.x PMid:22512503

Shi S, Bartold PM, Miura M, et al. The efficacy of mesenchymal stem cells to regenerate and repair dental structures. Orthod Craniofac Res. 2005; 8(3):191-199. https://doi.org/10.1111/j.1601-6343.2005.00331.x PMid:16022721

Ikeda H, Sumita Y, Ikeda M, et al. Engineering bone formation from human dental pulp- and periodontal ligament-derived cells. Ann Biomed Eng. 2011; 39(1):26-34. https://doi.org/10.1007/s10439-010-0115-2 PMid:20614244

Fawzy El-Sayed KM, Paris S, Becker ST, et al. Periodontal regeneration employing gingival margin-derived stem/progenitor cells: an animal study. J Clin Periodontol. 2012; 39(9):861-870. https://doi.org/10.1111/j.1600-051X.2012.01904.x PMid:22694281

Wang F, Yu M, Yan X, et al. Gingiva-derived mesenchymal stem cell-mediated therapeutic approach for bone tissue regeneration. Stem Cells Dev. 2011; 20(12):2093- 2102. https://doi.org/10.1089/scd.2010.0523 PMid:21361847

Weinberg E, Maymon T, Weinreb M. AGEs induce caspase-mediated apoptosis of rat BMSCs via TNFα production and oxidative stress. J Mol Endocrinol. 2013; 52(1):67-76.

Donath MY, Shoelson SE. Type 2 diabetes as an inflammatory disease. Nat Rev Immunol. 2011; 11(2):98-107. https://doi.org/10.1038/nri2925 PMid:21233852

Chen J, Song M, Yu S, et al. Advanced glycation endproducts alter functions and promote apoptosis in endothelial progenitor cells through receptor for advanced glycation endproducts mediate overpression of cell oxidant stress. Mol Cell Biochem. 2010; 335(1- 2):137-146.

Weinberg E, Maymon T, Weinreb M. AGEs induce caspase-mediated apoptosis of rat BMSCs via TNFalpha production and oxidative stress. J Mol Endocrinol. 2014; 52(1):67- 76. PMid:24198288

Rodrigues M, Wong VW, Rennert RC, Davis CR, Longaker MT, Gurtner GC. Progenitor Cell Dysfunctions Underlie Some Diabetic Complications. Am J Pathol. 2015. https://doi.org/10.1016/j.ajpath.2015.05.003 PMCid:PMC4607762

Cianfarani F, Toietta G, Di Rocco G, Cesareo E, Zambruno G, Odorisio T. Diabetes impairs adipose tissue-derived stem cell function and efficiency in promoting wound healing. Wound Repair Regen. 2013; 21(4):545-553. https://doi.org/10.1111/wrr.12051 PMid:23627689

Donath MY, Shoelson SE. Type 2 diabetes as an inflammatory disease. Nat Rev Immunol. 2011; 11(2):98-107. https://doi.org/10.1038/nri2925 PMid:21233852

Russo V, Yu C, Belliveau P, Hamilton A, Flynn LE. Comparison of human adipose- derived stem cells isolated from subcutaneous, omental, and intrathoracic adipose tissue depots for regenerative applications. Stem Cells Transl Med. 2014; 3(2):206-217. https://doi.org/10.5966/sctm.2013-0125 PMid:24361924 PMCid:PMC3925056

Ju L, Zhang X, Deng Y, et al. Enhanced expression of Survivin has distinct roles in adipocyte homeostasis. Cell Death Dis. 2017;8(1):e2533. https://doi.org/10.1038/cddis.2016.439

Ghorbani A, Mojarrad M, Hatami A, Hoseini S, Ghazavi H, Hosseini A. Effects of Streptozotocin-Induced Diabetes on Proliferation and Differentiation Abilities of Mesenchymal Stem Cells Derived from Subcutaneous and Visceral Adipose Tissues. Exp Clin Endocrinol Diabetes. 2016;125(1):33-41. https://doi.org/10.1055/s-0042-113460

Rennert RC, Sorkin M, Januszyk M, et al. Diabetes impairs the angiogenic potential of adipose-derived stem cells by selectively depleting cellular subpopulations. Stem Cell Res Ther. 2014;5(3):79. https://doi.org/10.1186/scrt468

Gao Y, Zhao G, Li D, Chen X, Pang J, Ke J. Isolation and multiple differentiation potential assessment of human gingival mesenchymal stem cells. Int J Mol Sci. 2014;15(11):20982-20996. https://doi.org/10.3390/ijms151120982

American Diabetes Association. Standards of Medical Care in Diabetes-2016 Abridged for Primary Care Providers. Clin Diabetes. 2016;34(1):3-21. https://doi.org/10.2337/diaclin.34.1.3

American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care. 2011;34 Suppl 1:S62-9. https://doi.org/10.2337/dc11-S062

Jumabay M, Moon JH, Yeerna H, Bostr??m KI. Effect of Diabetes Mellitus on Adipocyte-Derived Stem Cells in Rat. J Cell Physiol. 2015;230(11):2821-2828. https://doi.org/10.1002/jcp.25012

Wang F, Yu M, Yan X, et al. Gingiva-Derived Mesenchymal Stem Cell-Mediated Therapeutic Approach for Bone Tissue Regeneration. Stem Cells Dev. 2011;20(12):2093-2102. https://doi.org/10.1089/scd.2010.0523

Tsumanuma Y, Iwata T, Washio K, et al. Comparison of different tissue-derived stem cell sheets for periodontal regeneration in a canine 1-wall defect model. Biomaterials. 2011;32(25):5819-5825. https://doi.org/10.1016/j.biomaterials.2011.04.071

Iwata T, Yamato M, Tsuchioka H, et al. Periodontal regeneration with multi-layered periodontal ligament-derived cell sheets in a canine model. Biomaterials. 2009;30(14):2716-2723. https://doi.org/10.1016/j.biomaterials.2009.01.032

Yang H, Gao L-N, An Y, et al. Comparison of mesenchymal stem cells derived from gingival tissue and periodontal ligament in different incubation conditions. Biomaterials. 2013;34(29):7033-7047. https://doi.org/10.1016/j.biomaterials.2013.05.025

Tang L, Li N, Xie H, Jin Y. Characterization of mesenchymal stem cells from human normal and hyperplastic gingiva. J Cell Physiol. 2011;226(3):832-842. https://doi.org/10.1002/jcp.22405

Otabe K, Muneta T, Kawashima N, Suda H, Tsuji K, Sekiya I. Comparison of Gingiva, Dental Pulp, and Periodontal Ligament Cells from the Standpoint of Mesenchymal Stem Cell Properties. Cell Med. 2012;4(1):13-22. https://doi.org/10.3727/215517912X653319

Stolzing A, Sellers D, Llewelyn O, Scutt A. Diabetes induced changes in rat mesenchymal stem cells. Cells Tissues Organs. 2010;191(6):453-465. https://doi.org/10.1159/000281826

Xu T, Zhong L, Gan L, et al. Effects of LG268 on Cell Proliferation and Apoptosis of NB4 Cells. Int J Med Sci. 2016;13(7):517-523. https://doi.org/10.7150/ijms.15507

Published
2018-02-01
How to Cite
1.
Assem M, Kamal S, Sabry D, Soliman N, Aly RM. Preclinical Assessment of the Proliferation Capacity of Gingival and Periodontal Ligament Stem Cells from Diabetic Patients. Open Access Maced J Med Sci [Internet]. 2018Feb.1 [cited 2020Nov.29];6(2):254-9. Available from: https://www.id-press.eu/mjms/article/view/oamjms.2018.076
Section
A - Basic Science