Preclinical Assessment of the Proliferation Capacity of Gingival and Periodontal Ligament Stem Cells from Diabetic Patients

  • Mostafa Assem National Research Centre, Cairo
  • Samia Kamal Cairo University Faculty of Oral and Dental Medicine, Cairo
  • Dina Sabry Cairo University, Kasr Alainy Faculty of Medicine, Cairo
  • Nadia Soliman National Research Centre, Cairo
  • Riham M. Aly National Research Centre, Cairo
Keywords: Proliferation, Survivin, Gingival Stem cells, Periodontal Ligament Stem Cells, Diabetes


BACKGROUND: Stem cells have recently received great interest as potential therapeutics alternative for a variety of diseases. The oral and maxillofacial region, in particular, encompasses a variety of distinctive mesenchymal (MSC) populations and is characterized by a potent multilineage differentiation capacity.

AIM: In this report, we aimed to investigate the effect of diabetes on the proliferation potential of stem cells isolated from controlled diabetic patients (type 2) and healthy individuals.

SUBJECTS & METHODS: The proliferation rate of gingival and periodontal derived stem cells isolated from diabetic & healthy individuals were compared using MTT Assay. Expression levels of Survivin in isolated stem cells from all groups were measured by qRt - PCR.

RESULTS: There was a significantly positive correlation between proliferation rate and expression of Survivin in all groups which sheds light on the importance of Survivin as a reliable indicator of proliferation. The expression of Survivin further confirmed the proliferation results from MTT Assay where the expression of stem cells from non - diabetic individuals was higher than diabetic patients. Conclusion: Taking together all the results, it could be concluded that PDLSC and GSC are promising candidates for autologous regenerative therapy due to their ease of accessibility in addition to their high proliferative rates.


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How to Cite
Assem M, Kamal S, Sabry D, Soliman N, Aly RM. Preclinical Assessment of the Proliferation Capacity of Gingival and Periodontal Ligament Stem Cells from Diabetic Patients. Open Access Maced J Med Sci [Internet]. 2018Feb.1 [cited 2020Nov.29];6(2):254-9. Available from:
A - Basic Science