Induction of Immune Responses by DNA Vaccines Formulated with Dendrimer and Poly (Methyl Methacrylate) (PMMA) Nano-Adjuvants in BALB/c Mice Infected with Leishmania major

  • Fatemeh Tabatabaie Department of Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences, Tehran
  • Nasim Samarghandi Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan
  • Somayeh Zarrati Microbiology Department, Science & Research Branch, Islamic Azad University, Tehran
  • Fatemeh Maleki 4Department of Parasitology, Faculty of Para Medical Sciences, Iran University of Medical Sciences, Tehran
  • Mehdi Shafiee Ardestani Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran
  • Taher Elmi Department of Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences, Tehran
  • Sayed Hussain Mosawi Department of Biology and Microbiology, Faculty of Medical Technology. Khatam Al Nabieen University, Kabul
Keywords: PMMA, Dendrimer, Leishmaniasis, TSA

Abstract

BACKGROUND: Leishmaniasis is a parasitic disease induced by a protozoan from the genus Leishmania. No effective vaccine has yet been developed against the disease.

AIM: In this work, two nano-vaccines, TSA recombinant plasmid and dendrimer and poly (methyl methacrylate) (PMMA) nanoparticles (as adjuvants), were designed and tested for their immunogenicity in BALB/c mice.

METHODS: After the plasmid construction and preparation of adjuvants, three intramuscular injections of the nano-vaccines (100 µg) and the recombinant TSA protein (20 µg) were subcutaneously performed. Eventually, the challenged animals were infected with the parasites (1*106 promastigotes). After the last injections of the nano-vaccines, the responses of their antibody subclasses and cytokines were assessed via ELISA method before and after the challenge.

RESULTS: This study revealed that the new nano-vaccines were strong and effective in inducing specific antibody and cellular responses and reducing the parasite burden in the spleen compared to the control groups of Leishmania major-infected BALB/c mice.

CONCLUSION: Based on the results, we can suggest that the formulated vaccines are suitable candidates for further studies in the field of leishmaniasis control.
 

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Published
2018-01-27
How to Cite
1.
Tabatabaie F, Samarghandi N, Zarrati S, Maleki F, Ardestani MS, Elmi T, Mosawi SH. Induction of Immune Responses by DNA Vaccines Formulated with Dendrimer and Poly (Methyl Methacrylate) (PMMA) Nano-Adjuvants in BALB/c Mice Infected with Leishmania major. Open Access Maced J Med Sci [Internet]. 2018Jan.27 [cited 2020Nov.25];6(2):229-36. Available from: https://www.id-press.eu/mjms/article/view/oamjms.2018.061
Section
A - Basic Science