Relation between microRNAs and Apoptosis in Hepatocellular Carcinoma

  • Refaat R. Kamel Surgery Department, Faculty of Medicine, Ain Shams University, Cairo
  • Khalda Said Amr Medical Molecular Genetics Department, National Research Center, Cairo
  • Mie Afify Biochemistry Department, National Research Centre, Cairo
  • Yasser A. Elhosary Internal Medicine Department, National Research Centre, Cairo
  • Abdelfattah E. Hegazy Surgery Department, Elsahel Teaching Hospital, Cairo
  • Hoda H. Fahim Anesthesia Department, Elsahel Teaching Hospital, Cairo
  • Wafaa M. Ezzat Internal Medicine Department, National Research Centre, Cairo
Keywords: HCC, apoptosis, pathogenesis, microRNAs, HCV

Abstract

AIM: To determine the relation between serum microRNAs and apoptotic markers as regards development of HCC to understand the underlying mechanism of HCV related hepatocarcinogenesis.

PATIENTS AND METHODS: A total of 65 serum samples (25 samples from controls, 20 samples from hepatitis and 20 samples from HCC patients) were collected for miRNAs (mir 21, mir 199-a, and mir 155) detection. Human Programmed cell death protein-4 (PDCD-4) and Human Cytochrome-C (CYT-C) were determined.

RESULTS: miRNAs 21 and 155 were over expressed in sera of patients with HCC compared to patients with chronic hepatitis (p < 0.0001). While serum means values of miR 199a was significantly decreased among HCC group patients when compared to patients with chronic hepatitis (p < 0.0001). The serum levels of PCDC4 and CYTC were increased in patients with HCC when compared to chronic hepatitis patients. They were also increased in patients with chronic hepatitis when compared to controls (p < 0.05, significant). There was direct correlations between apoptotic markers and oncomirs miRNAs 21 and 155 while apoptotic markers were inversely correlated with miRNA 199-a.

CONCLUSION: Both microRNAs and apoptotic markers have roles in HCC pathogenesis. It seems that oncogenic microRNAs induce liver carcinogenesis in HCV patients irrespective of suppression of apoptosis.

AIM: To determine the relation between serum microRNAs and apoptotic markers as regards development of HCC to understand the underlying mechanism of HCV related hepatocarcinogenesis.

PATIENTS AND METHODS: A total of 65 serum samples (25 samples from controls, 20 samples from hepatitis and 20 samples from HCC patients) were collected for miRNAs (mir 21, mir 199-a, and mir 155) detection. Human Programmed cell death protein-4 (PDCD-4) and Human Cytochrome-C (CYT-C) were determined.

RESULTS: miRNAs 21 and 155 were over expressed in sera of patients with HCC compared to patients with chronic hepatitis (p < 0.0001). While serum means values of miR 199a was significantly decreased among HCC group patients when compared to patients with chronic hepatitis (p < 0.0001). The serum levels of PCDC4 and CYTC were increased in patients with HCC when compared to chronic hepatitis patients. They were also increased in patients with chronic hepatitis when compared to controls (p < 0.05, significant). There was direct correlations between apoptotic markers and oncomirs miRNAs 21 and 155 while apoptotic markers were inversely correlated with miRNA 199-a.

CONCLUSION: Both microRNAs and apoptotic markers have roles in HCC pathogenesis. It seems that oncogenic microRNAs induce liver carcinogenesis in HCV patients irrespective of suppression of apoptosis.

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Published
2016-03-09
How to Cite
1.
Kamel RR, Amr KS, Afify M, Elhosary YA, Hegazy AE, Fahim HH, Ezzat WM. Relation between microRNAs and Apoptosis in Hepatocellular Carcinoma. Open Access Maced J Med Sci [Internet]. 2016Mar.9 [cited 2020Nov.25];4(1):31-7. Available from: https://www.id-press.eu/mjms/article/view/oamjms.2016.038
Section
A - Basic Science