Role of Cardiac Myocytes Heart Fatty Acid Binding Protein Depletion (H-FABP) in Early Myocardial Infarction in Human Heart (Autopsy Study)

  • Amany Shabaiek Egyptian Forensic Medicine Authority, Pathology Department, Cairo
  • Nour El-Hoda Ismael Faculty of Medicine, Kasr El- Aini, Pathology Department, Cairo
  • Samar Elsheikh Faculty of Medicine, Kasr El- Aini, Pathology Department, Cairo
  • Hebat Allah Amin Egyptian Forensic Medicine Authority, Pathology Department, Cairo
Keywords: detection myocardial infarction, H-FABP, immunohistochemistry, MI, sudden cardiac death

Abstract

BACKGROUND: Many immunohistochemical markers have been used in the postmortem detection of early myocardial infarction.

AIM: In the present study we examined the role of Heart-type fatty acid binding protein (H-FABP), in the detection of early myocardial infarction.

MATERIAL AND METHODS: We obtained samples from 40 human autopsy hearts with/ without histopathological signs of ischemia.

RESULTS: All cases of definite and probable myocardial infarction showed a well-defined area of H-FABP depletion. All of the control cases showed strong H-FABP expression, except two markedly autolysed myocardial samples that showed affected antigenicity.

CONCLUSION: Thus, we suggest H-FABP as being one of the valuable tools facing the problem of postmortem detection of early myocardial infarction/ischemia, but not in autolysis.

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References

Hashmi S, Al-Salam S. Loss of dystrophin staining in cardiomyocytes: a novel method for detection early myocardial infarction. Int J Clin Exp Pathol. 2013;6(2):249-57.

PMid:23330010 PMCid:PMC3544224

Bouchardy B, Majno G. Histopathology of early myocardial infarcts. A new approach. The American Journal of pathology. 1974;74: 301-30.

PMid:4359735 PMCid:PMC1910768

Hansen SH, Rossen K. Evaluation of cardiac troponin I immunoreaction in autopsy hearts: a possible marker of early myocardial infarction. Forensic Science International. 1999;99(3):189- 196.

http://dx.doi.org/10.1016/S0379-0738(98)00193-5

Fishbein MC, Wang T, Matijasevic M, Hong L, Apple FS. Myocardial tissue troponins T and I. An immunohistochemical study in experimental models of myocardial ischemia. Cardiovasc Pathol. 2003;12(2):65-71.

http://dx.doi.org/10.1016/S1054-8807(02)00188-6

Martínez Díaz F, Rodríguez-Morlensín M, Pérez-Cárceles MD, Noguera J, Luna A, Osuna E. Biochemical analysis and immunohistochemical determination of cardiac troponin for the postmortem diagnosis of myocardial damage. Histol Histopathol. 2005;20(2):475-81.

PMid:15736052

Saukko P, Knigh B. The Pathology of Sudden Death Forensic Pathology. Third Edition, Arnold. London, 2004:488-492.

Servonnet A, Delacour H, Dehan C, Gardet V. [Heart fatty-acid binding protein (h-FABP): a new cardiac marker]. Ann Biol Clin (Paris). 2006;64(3):209-17.

Ming M, Meng XZ. Immunohistochemical observation of heart-type fattyacid binding-protein in early myocardial ischemia. Europe Pubmed Central. 2004;20(1):15-17.

Meng X, Ming M, Wang E. Heart fatty acid binding protein as a marker for postmortem detection of early myocardial damage. Forensic Sci Int. 2006;160(1):11-6.

http://dx.doi.org/10.1016/j.forsciint.2005.08.008

PMid:16182485

LODGE PATCH I. The ageing of cardiac infarcts, and its influence on cardiac rupture. British Heart Journal. 1951;13:37–42.

http://dx.doi.org/10.1136/hrt.13.1.37

PMid:14791881 PMCid:PMC503664

Amin HAA, El-Hennawy AMY, Nakhla GAA, Tabak SA, Hassan HH. Immunohistochemistry in the detection of early myocardial infarction - A post-mortem study. Egyptian Journal of Forensic Sciences. 2011;(1):5–12.

Kenichi Y. Pursuing enigmas on ischaemic heart disease and sudden cardiac death. Legal Medicine. 2009;11:51–58.

http://dx.doi.org/10.1016/j.legalmed.2008.08.005

PMid:19042146

Campobasso CP, Dell'Erba AS, Addante A, Zotti F, Marzullo A, Colonna MF. Sudden cardiac death and myocardial ischemia indicators: a comparative study of four immunohistochemical markers. Am J Forensic Med Pathol. 2008;29(2):154-61.

http://dx.doi.org/10.1097/PAF.0b013e318177eab7

PMid:18520484

Ribeiro-Silva A, S Martin CC, Rossi MA. Is immunohistochemistry a useful tool in the postmortem recognition of myocardial hypoxia in human tissue with no morphological evidence of necrosis? Am J Forensic Med Pathol. 2002;23(1):72-7.

http://dx.doi.org/10.1097/00000433-200203000-00016

PMid:11953500

Bi H, Yang Y, Huang J, Li Y, Ma C, Cong B. Immunohistochemical detection of S100A1 in the postmortem diagnosis of acute myocardial infarction. Diagn Pathol. 2013;8:84.

http://dx.doi.org/10.1186/1746-1596-8-84

PMid:23683996 PMCid:PMC3663776

Amin HAA, Abdelal HA, Shabaiek AA, Osman GS, Mousa AA. A comparative study of immunohistochemical markers in the detection of early myocardial infarction (Autopsy study). Egyptian journal of pathology. 2015;35(1):76-80.

http://dx.doi.org/10.1097/01.XEJ.0000465414.47169.95

Published
2016-01-21
How to Cite
1.
Shabaiek A, Ismael NE-H, Elsheikh S, Amin HA. Role of Cardiac Myocytes Heart Fatty Acid Binding Protein Depletion (H-FABP) in Early Myocardial Infarction in Human Heart (Autopsy Study). Open Access Maced J Med Sci [Internet]. 2016Jan.21 [cited 2020Dec.5];4(1):17-1. Available from: https://www.id-press.eu/mjms/article/view/oamjms.2016.018
Section
A - Basic Science