The Study of Cellular and Molecular Physiological Characteristics of Sperm in Men Living in the Aral Sea Region

  • Berikbay Z. Kultanov Karaganda State Medical University, Department of the Republic of Kazakhstan, Gogolya street 40, Karaganda
  • Raushan S. Dosmagambetova Karaganda State Medical University, Department of the Republic of Kazakhstan, Gogolya street 40, Karaganda
  • Svetlana A. Ivasenko Karaganda State Medical University, Department of the Republic of Kazakhstan, Gogolya street 40, Karaganda
  • Yelena S. Tatina Karaganda State Medical University, Department of the Republic of Kazakhstan, Gogolya street 40, Karaganda
  • Assel A. Kelmyalene Karaganda State Medical University, Department of the Republic of Kazakhstan, Gogolya street 40, Karaganda
  • Lyazzat H. Assenova Karaganda State Medical University, Department of the Republic of Kazakhstan, Gogolya street 40, Karaganda
Keywords: Aral Sea, ASF, RNA, DNA, H1, H2A, H3, H4

Abstract

BACKGROUND: Extreme environmental situation in the Aral crisis has caused a massive chemical pollution of the territory for decades with high doses of pesticides, herbicides. Discharge of industrial waste into the rivers that feed the Aral Sea has lead to the development of various pathological processes in the human body, as well as disruption of reproductive function in young men.

AIM: To evaluate the performance of molecular cellular changes in the sperm of men under the influence of dust and salt aerosols in Aral Sea region.

MATERIAL AND METHODS: Clinical and laboratory studies were conducted in men 5 settlements (Aralsk-city, v. Aiteke-Bi, v. Zhalagash, v. Zhusaly, v. Shieli). We have studied male ejaculate obtained after 4-5 days of abstinence, and placed it in a warm tube with a glass stopper. On the investigation proceeded ejaculate within 20-30 minutes after its preparation, during which time he was subjected to liquefaction. Isolation and quantification of ASF, RNA, DNA, and determining the fraction of histones in sperm was performed by the method of Markusheva and Savina.

RESULTS: It was found that the value of ASF in the semen of men living in the zone of ecological disaster higher compared with the values of parameters in men living in the area of environmental crisis, and this trend is observed in all age groups. The study of circulating extracellular DNA and RNA in the sperm of men registered their decline with a corresponding increase of acid precursors that can be attributed to the degradation of nucleic acids under the influence of negative factors in the complex area of ecological trouble. Also, according to a study in men residing in the areas of environmental catastrophe at the age of 18-29 years, found an increased content of the H1 histone H2A lower total fraction, H3, H4 - and a sharp increase in histone H2B content - histones.

CONCLUSIONS: Men living in environmentally disadvantaged areas of Kyzylorda region under the influence of dust and salt aerosols and other toxicants leads to disruption of the reproductive function in men.

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References

Astankulov KM, Annamukhamed MB, Astankulov RS Kurbanov NR et al. Comparative evaluation of indicators of actual pesticide load the body of people living in areas of intensive and low-intensity use of pesticides. Health Turkmenistan. 1990;3:26-30.

Mikhailov VN, Kravtsov V, Gurov FN, Markov DV, Gregoire M. Evaluation of the current state of the Aral Sea. Vestnik MGU Ser Geography. 2001;6:14-21.

Baizhanova MM, Imankulova KD. The structure and the prevalence of lung diseases in children. Health and disease. 2006;4:74-78.

Gabdreeva GI. Health of the population living in the area of environmental pre-crisis state. Health of Kazakhstan. 2005;4:17-19.

Tkachenko NV, Bogdanova MI, Yakovleva NA. Comparative characteristics of immunological parameters in women of childbearing age of large industrial cities of Kazakhstan. Medi ecology. 2006;2:30-33.

Bozhedomov VA, Rohlikov IM, Tretyakov AA, Lipatov NA, Vinogradov IV. Andrology aspects of childless marriage. Medical Council. 2013;8(1):13-17.

Markusheva LI, Savina MI, Reshin VM et al. Clinical Laboratory. 2000;7:18-20.

Tatina YS, Dyusesenbekova BN, Kislitskaya VN, Kenzhin ZhD, Estemesova KA, Yessilbayeva BT, Kultanov BZh. Assesing the level of medium-weight molecules in the semen of men of reproductive age in the area of environmental crisis of Aral Sea region. The Febs Journal. 2015;282(1):118.

Vinogradova LA, Savina LV, Parkhomenko TK. Risk assessment of the impact of adverse environmental factors on the results of sanitary and microbiological and clinical studies. Hygiene and sanitation. 1990;12:7-8.

Zhumatova MG, Lokshin VN. Problems of women's reproductive health in Kazakhstan. Problems of reproduction. 2010;3:24-27.

Published
2015-12-22
How to Cite
1.
Kultanov BZ, Dosmagambetova RS, Ivasenko SA, Tatina YS, Kelmyalene AA, Assenova LH. The Study of Cellular and Molecular Physiological Characteristics of Sperm in Men Living in the Aral Sea Region. Open Access Maced J Med Sci [Internet]. 2015Dec.22 [cited 2020Dec.5];4(1):5-. Available from: https://www.id-press.eu/mjms/article/view/oamjms.2016.007
Section
A - Basic Science